Cervical Cytology
Normal cell content of cervical smears, contaminants and artifacts. Adequacy and unsatisfactory smears
Normal Cell Content
Basic Smear Patterns
Smear adequacy: The unsatisfactory smear

 

Specimen adequacy

  • It is important to remember that, like all screening test the cervical smear test is not 100% effective in detecting abnormalities present in the cervix.
  • The reasons for  failure of the Pap test to contain abnormal cells in the presence of a histologically  proven preneoplastic or neoplastic lesion can be attributed to:
    1. The biological properties of the tumour
    2. Failure of sampling
    3. Failure of screening
Biological properties of the tumour:
 

Studies have shown that small CIN lesions (< 0.5cm diameter) and lesions that are high in the endocervical canal  are more likely to be missed . Regular smear tests at 3 or 5 yearly intervals will  minimise the risk  of missing CIN  lesion when they are in the earliest stages of development.


Failure of sampling:
 

A neoplastic lesion may be missed if the whole of the transformation zone is not sampled. This can happen if the sampling device selected by the smear taker  is not appropriate for the shape or size of the cervix,  A neoplastic lesion may also be missed if abnormal cells present on the sampling device are not transferred to the slide. Great care should be taken by the smear taker to ensure that as much material as possible  is transferred from spatula to slide. It is anticipated that  the use of Liquid based cytology will resolve this problem.


Failure of screening:
 

A false negative report may be issued if the primary screener fails to detect abnormal  cells in the smear.  This risk can be minimised if appropriate quality control measures are in place in the laboratory (LINK to module on  Quality Control).

Evaluation of specimen adequacy by the screener is considered by many to be the single most important Quality Assurance procedure that can be provided by the laboratory

Definition of adequacy

  • A conventional smear can be considered to be satisfactory for evaluation if it has an estimated minimum of 8000 -12000 well preserved clearly displayed squamous epithelial cells. Cellularity can be estimated against reference images (see The Bethesda System for reporting cervical cytology: (second edition)) but this is not a rigid threshold.The woman’s age and menstrual status must be taken into account when assessing adequacy
  • Data on the significance of transformation zone component are conflicting.The most recent studies fail to show an association between evidence of T/Z sampling and high grade cervical lesions.  The presence of endocervical cells and/or  immature metaplastic cells are not per se reliable indicators of smear adequacy.
  • Liquid Based Cytology: Liquid based preparations can be considered to be satisfactory for evaluation provided they have  an estimated minimum of at least 5000 well visualised, well preserved squamous cells.
  • A cervical smear or LBC preparation  must not be regarded as inadequate if abnormal cells are present.

Inadequate smears

Slides may be unsatisfactory for evaluation for any of  the following reasons:

  • If the degree of cellularity is insufficient taking into account the woman’s age and hormone status
  • If the smear is entirely composed of of separated superficial cells suggesting a vaginal rather than a cervical origin
  • If it is poorly fixed or air dried to such a degree that assessment is impossible
  • If the cellular material is thickly spread or is obscured by blood , debris , polymorphs ,bacteria  or sperm
  • If it is entirely composed of endocervical cells unless the only object of the test was to sample the endocervical mucosa.
  • A cervical smear or LBC preparation  must not be reported  as inadequate if abnormal cells are present
  • Colposcopy is advisable after three consecutive inadequate smears
Inadequate smears
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